Crystallization Process Development II: Peptide Crystallization

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Crystallization Process Development II: Peptide Crystallization

Solving the challenges of peptide crystallization yields downstream rewards

Hayley Reece, Executive Director of Technical Services, Solid Form Solutions Ltd, an Avista Pharma Company
Dr. Laura Allan, Senior Scientist, Solid Form Solutions Ltd, an Avista Pharma Company

A scalable solution: Are peptides worth the extra effort?

Peptides play an increasingly important role in drug discovery with their potential to offer therapeutic agents beyond traditional hormone replacement therapies. In addition to therapeutic agents, peptides are often subcomponents in other drug substances. Regardless of the application, mastering peptide crystallization opens possibilities for large scale processing in pharma applications.

The molecular complexity of peptides may have kept them out of development pipelines in the
past, but innovations in synthesis and purification have supported their recent momentum as alternatives to small molecules for addressing unmet medical needs. Still, peptides present a particularly challenging class of molecules in crystallization process development. Success of scale-up crystallizations relies on a robust crystallization process
to control the thermodynamic and kinetic factors that impact the solid form. Peptide Crystallization

Given the difficulty of peptide crystallization, why do companies invest the time and resources into pursuing a crystalline form of their peptide? Having a crystalline solid form offers clear advantages compared to their amorphous counterparts. Not only does it allow full structural determination, but it also confers benefits in processing of the material. Crystalline products tend to be less hygroscopic than amorphous materials, aiding in further processing. Significant impurity rejection and a greater ease of residual solvent removal is afforded through crystallization, resulting in a higher purity product. Furthermore, peptide crystallization offers a significant cost advantage over column chromatography methods for isolation and purification of the desired solid form.

Obtaining a pure crystalline form of any API is exacting work, and peptides are a special case within this area of chemistry. Before scientists can consistently produce a stable solid form, they must have an in-depth understanding of the thermodynamic and kinetic processes that drive the crystallization fora specific peptide. Only then can they manipulate various critical process parameters to yield the optimal result. Solid Form Solutions Ltd, an Avista Pharma company, employs world class experts in the understanding of crystal formation and thermodynamics, allowing us to develop custom scalable crystallization processes for peptides. Our combined experience ensures that we conduct the right experiments and modify parameters to achieve the optimal crystalline form. Whether it is performed during formation of an API or as a means to purify intermediates, achieving control of nucleation and crystal growth is critical to designing a successful peptide crystallization process.

Milestones to success: 3 stages of strategy

For peptide crystallization to be successful, the fine tuning of several process conditions is critical. Typically, operating ranges for peptide crystallization are much tighter than for small molecules, so the design and assessment of initial screening can make or break scale-up efforts.

Solid Form Solutions Ltd (SFS) meets 3 milestones during their overall strategy to deliver crystallized peptides. Solubility is evaluated with respect to solvents, pH, temperature, counterion inclusion and excipients. This serves a dual purpose of finding conditions under which the peptide is soluble and discovering the optimal conditions for later crystallization. Small-scale crystallization trials are then carried out to locate experimental conditions that allow for crystallization of the free peptide or its salt form. A wide range of conditions are considered, in order to avoid gelation, aggregation, amorphous solids and liquid crystals. Achieving single crystal growth during these first steps allows structural analysis and provides material for seeding protocols.

Next, the team looks for scalable elements of the process. At this point, they assess the critical process parameters at the 250 mL or 1 L scale. The conditions are adjusted to achieve the best yield, with the highest purity and efficiency in filtration, in a minimal reaction volume. At the same time, they must define the operating range for each of the critical process factors to deliver a controlled and scalable crystallization.

The third milestone involves technology transfer to ensure a successful crystallization process in the client’s facility. Our experts transfer the process to achieve consistent results in the plant using data and analysis from hands-on experiments as well as state-of-the-art modeling software.

Significant uplift in purity: Preferred conditions

Solubility of the peptide can be affected by several factors, including pH, counterion inclusion, temperature, and solvent selection. Once solubility curves are established, a screening array can be designed to further define crystallization conditions and obtain first crystals for analysis.

In the case discussed here, the team started with an amorphous lyophilized solid with 89% w/w purity that had low stability at high pH and sensitivity to light and heat. The goal was to increase purity to greater than 98% w/w. An array of experimental conditions investigated various organic solvents, pH levels, peptide concentrations, and multiple temperatures. For this peptide, the most effective crystallization occurred with low starting pH, using an organic solvent precipitant, with counter-ion inclusion and a temperature of 20°C. After these steps of optimization, a purity uplift to 97% w/w was observed from the initial crystallization.

The team then set out to determine the critical crystallization process parameters to ensure optimal operating ranges during scale-up. Using controlled laboratory reactors equipped with an online FBRM probe, the team measured several parameters in situ, including temperature, cooling profiles, precipitant addition rate and volume, changes in concentration, aging periods, effect of seeding, counter-ion concentrations, and mixing effects. SFS exceeds industry standards in online monitoring using next generation technology. The Blaze Metrics 3-in-1 PAT tool allows sophisticated real-time monitoring of particle shape, size and polymorphic form. It also allows SFS to offer clients reduced set-up time with a single probe, while providing state-of-the-art insight into particle and process behavior.

FBRM probeIn this case, the team determined several parameters that were deemed most critical for successful crystallization of the studied peptide. Follow up experiments separately investigated conditions including peptide concentration, aqueous to organic solvent ratio, counter-ion concentration, and solvent addition rate under various pH conditions and temperatures. They also demonstrated that changes in peptide concentration had little effect on the crystallization yield or purity. While seeding was not required to obtain the final crystalline material, the team’s analysis showed that it resulted in an increased particle size, improving the filtration rate.

Next, the team performed washing and studies to ensure that the crystalline nature of the peptide would be maintained upon isolation. This can be a critical step during scale-up to avoid deliquescence of the peptide solid on the filter. In this case, a range of organic/aqueous solvent mixtures were tested for optimal washing, and it was determined that organic solvent without an aqueous component allowed a final flowable solid to be isolated, avoiding loss of material as an oil residue to the filter.

With these parameters in place, the process was carried out in a 1 L reactor, and the team was able to successfully produce a purified, flowable solid with a purity increase from 89% w/w to 99.6% w/w. Success at the 1 L scale allowed method transfer to begin at the client site, with full support during the process to ensure a smooth transfer and consistent replication in production of the crystalline solid form.


Process design innovation: No substitute for expertise

While crystallization efforts are traditionally associated with small molecules, the step-wise approach discussed here is a successful strategy to tackle the design of a peptide crystallization process, adding to Avista Pharma’s command in this space of drug development. The increased understanding provided by these studies is a powerful tool forPeptide Crystallization our clients to assess the potential of their peptide and to reveal its viability as a candidate for crystallization. Knowing whether the properties of a specific crystallized peptide will offer advantages over the amorphous material can prevent wasted scale-up efforts.

Achieving control of crystal nucleation and growth through in-depth analysis of critical process parameters is key to being able to transfer a bench-scale process to meet clinical demand. This example highlights that strategic design principles coupled with leading experts and technology can lead to successful scale-up of even the most stubborn peptide crystallizations. We leverage the best minds in the industry to replicate laboratory-scale crystallization in large scale reactors, helping our clients achieve clinical delivery timelines.

Success beyond science.

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